Stimulation of lignin synthesis via the PAL-associated metabolic pathway in wheat plants (Tríticum aestívum L.)

Pavel Feduraev,Artem Pungin,Elina Tokupova,Liubov Skrypnik,Anastasiia Riabova

doi:10.1051/e3sconf/202129102017

Pavel Feduraev, Artem Pungin + Show 3 more

Open Access

https://doi.org/10.1051/e3sconf/202129102017

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Abstract

The paper demonstrates an approach to increasing the accumulation of lignin in plants of soft wheat (Tríticum aestívum L.) at the early stages of ontogenesis, by means of substrate stimulation of the key enzyme of the synthesis of phenylpropanoids – phenylalanine-ammonia-lyase. It was shown that plants grown on nutrient media containing phenialanine or tyrosine at a concentration of 500 μM significantly increased the lignin content of cell walls compared to controls.

Highlights

One of the principle crops, both in our country and around the world, is wheat (Tríticum aestívum L.)
Considering the above, the goal of this study is to evaluate the role of aromatic amino acids phenylalanine and tyrosine in lignin accumulation in wheat plants (Triticum aestivum L.)
There was a significant (p ≤ 0.05) increase in the phenylalanine ammonia-lyase (PAL) and TAL activity compared to the control, but only when the results were normalized for the corresponding products of enzymatic transformation (PAL for trans-cinnamic acid (TCA) and TAL for p-coumaric acid (PCA))

Summary

Introduction

One of the principle crops, both in our country and around the world, is wheat (Tríticum aestívum L.). The most typical agronomic practice in wheat cultivation remains monoculture cultivation. This type of cultivation is associated with a number of negative effects, and primarily with a decrease in plant resistance to both biotic and abiotic environmental factors. Lignin is a phenolic polymer that is mainly deposited in the secondary cell wall during the last stages of cellular differentiation. It displaces the water phase of cell wall, covering the cellulose and matrix polysaccharides, and provides increased mechanical strength and a watertight barrier of plant cell [1]. The importance of lignin in these tissues is demonstrated by vascular collapse in lignindeficient plants [2]

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