Abstract
Abstract Studies were performed to characterize some of the stimulatory effects of several short-chain alcohols on adenyl cyclase (AC) in rat liver, intestine, brain, and adipose tissue, and in human intestinal tissue. It was found that the AC activity in the rat was stimulated by ethanol to some degree in all of the tissues which were measured, but ethanol and methanol caused more AC activity in the rat intestine than did the fluoride ion. The rat liver showed maximal alcohol stimulation only after treatment with Lubrol WX. Human, intestinal AC was also stimulated by ethanol and methanol, but to a lesser degree than in the rat tissues. The product formed from the reaction of the tissue fractions with ATP was identified as cyclic 3′,5′-cyclic adenosine monophosphate (cAMP) by thin layer chromatography and phosphodiesterase reaction. No cAMP was formed if the AC tissue fraction was boiled for 5 minutes prior to incubation. The mechanism by which the alcohols stimulate the AC, or whether ethanol stimulates the AC in vivo is not known.
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