Abstract
Dental pulpal infection is most commonly caused by extensive dental caries. A principal driving force behind pulpal disease response appears to lie in the immune system's response to bacteria. However, the production of interleukin (IL)-1β and IL-6 in human dental pulp (HDP) cells in response to lipopolysaccharide (LPS) has not been well characterized. We examined IL-1β and IL-6 production in HDP cells by challenging with LPS fromPorphyromonas endodontalis,which is a Gram-negative bacteria found in root canals. Our results presented here showed that when HDP cells were stimulated by LPS, the production of IL-6 always preceded that of IL-1β. Since the IL-6 production was observed even in the presence of the IL-1β receptor antagonist, we concluded IL-6 production was independent of the IL-1β molecule in LPS-stimulated HDP cells. This idea was further supported by the results obtained from RT-PCR experiments, in which IL-6 mRNA, but not IL-1β mRNA, was present in the RNA preparation isolated from the early stage of cells.
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