Stimulation of Innate and Adaptive Immunity by Using Filamentous Bacteriophage fd Targeted to DEC-205.

Luciana D’Apice,Marianna Aprile,Piergiuseppe De Berardinis,Valerio Costa,Maria Trovato,Rossella Sartorius

doi:10.1155/2015/585078

Abstract

The filamentous bacteriophage fd, codisplaying antigenic determinants and a single chain antibody fragment directed against the dendritic cell receptor DEC-205, is a promising vaccine candidate for its safety and its ability to elicit innate and adaptive immune response in absence of adjuvants. By using a system vaccinology approach based on RNA-Sequencing (RNA-Seq) analysis, we describe a relevant gene modulation in dendritic cells pulsed with anti-DEC-205 bacteriophages fd. RNA-Seq data analysis indicates that the bacteriophage fd virions are sensed as a pathogen by dendritic cells; they activate the danger receptors that trigger an innate immune response and thus confer a strong adjuvanticity that is needed to obtain a long-lasting adaptive immune response.

Highlights

Vaccines are one of the most successful outcomes of modern medicine in improving the global health
We have demonstrated that this doubledisplaying bacteriophage induces stronger antigenic response if compared to nontargeted bacteriophage, enhancing uptake by dendritic cells and inducing DC maturation [11]
Using a system vaccinology approach based on RNASequencing analysis of bone marrow derived dendritic cells (BMDCs) pulsed with fdsc-αDEC, here we report new insights about the molecular mechanisms by which filamentous bacteriophage induces protective immunity

Summary

Introduction

Vaccines are one of the most successful outcomes of modern medicine in improving the global health. Many diseases are still a challenge for vaccine development and the attempt to make new vaccines using more recent technologies has required the use of adjuvants, which enhance the magnitude and modulate the quality of the immune response. In this context, recent failure in producing functional vaccines against emerging diseases has shown that formulating a vaccine able to induce a protective immunity should involve the innate immunity. The incorporation of pathogen associated with molecular patterns (PAMPs) in vaccine formulations can improve and accelerate the induction of vaccine-specific responses

Methods

Results

Discussion

Conclusion