Abstract
Ecdysterone has been shown to stimulate hydrocarbon biosynthesis in Sarcophaga bullata at pupariation. When post-feeding larva were treated with 3H-acetate 10·5 hr after hormone administration, a 1·3 times greater quantity of 3H-acetate was incorporated into hydrocarbon in the ecdysterone injected insects than controls. A similar experiment with a 24 hr delay of 3H-acetate administration following hormone treatment resulted in 3·3 times greater incorporation into hydrocarbon of treated animals. Isolated integuments synthesize hydrocarbon from acetate better than internal tissues, and the integuments of ecdysterone-treated insects incorporate acetate into hydrocarbon 9·8 times better than integuments of control insects. This indicates that cuticular hydrocarbon biosynthesis not only occurs in the integument, but that a locus of regulation is present in the integument.
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