Stimulation of fatty acid biosynthesis in glycine max suspension cultures by acyl carrier protein

Abstract

Fatty acid synthesis in disrupted proplatids of Glycine max (soybean) cell suspension cultures is stimulated by E. coli acyl carrier protein (ACP). From a cell free homogenate of G. max cultures the fatty acid synthetase was precipitated by ammonium sulfate fractionation between 30–70% saturation. In this fraction, fatty acid synthesis, as determined by incorporation of malonylCoA into fatty acids, was enhanced by the addition of E. coli ACP and, to a lesser degree, by an acid precipitate obtained from the supernatant of the G. max cell free homogenate saturated with 70% ammonium sulfate.