Abstract
Fatty acid synthesis in disrupted proplatids of Glycine max (soybean) cell suspension cultures is stimulated by E. coli acyl carrier protein (ACP). From a cell free homogenate of G. max cultures the fatty acid synthetase was precipitated by ammonium sulfate fractionation between 30–70% saturation. In this fraction, fatty acid synthesis, as determined by incorporation of malonylCoA into fatty acids, was enhanced by the addition of E. coli ACP and, to a lesser degree, by an acid precipitate obtained from the supernatant of the G. max cell free homogenate saturated with 70% ammonium sulfate.
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