Abstract
The effect of vanadate on ethylene biosynthesis in detached rice leaves was investigated. Vanadate at pH 5.0–7.0 effectively enhanced ethylene production within 3 h of its application. It promoted the conversion of ACC to ethylene. Treatment with vanadate did not decrease ACC level until late stage of incubation, i.e. at 12 h after incubation. Molybdate, an inhibitor of phosphatase had no or much less stimulatory effect on ethylene production than did vanadate at comparable concentrations. Azide, an inhibitor of F1-ATPase, inhibited ethylene production in detached rice leaves. FC and vanadate were observed to be synergisticly increased ethylene production in detached rice leaves. In conclusion, plasma membrane H+-ATPase does not seem to be involved in ethylene biosynthesis in detached rice leaves.
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