Stimulation of DNA synthesis in human and hamster cells by human adenovirus types 12 and 5.

Abstract

SummaryThe effect of adeno-12 and adeno-5 viruses on the incorporation of thy-midine-H3 by cultures of human KB cells, newborn hamster kidney cells, and hamster embryo cells was investigated by radioautog-raphy. In KB cultures, infection with either adeno-5 or adeno-12 virus significantly increased the percentage of labelled cells, but the number of silver grains per labelled cell was somewhat lower than for control cultures. In hamster kidney cells, infection with either adeno-5 or adeno-12 decreased the mitotic index but significantly increased both the percentage of labelled cells and the intensity of label per labelled cell. Intranuclear viral inclusion bodies were formed in as high as 90% of the hamster kidney cells infected with adeno-5, which replicates in hamster cells. The incorporated label was closely associated with the intranuclear viral inclusion bodies. No viral inclusion bodies were found in the hamster kidney cells infected with adeno-12, which has an incomplete growth cycle in hamster ce...