Stimulation of Codonopsis pilosula Polysaccharide on Bifidobacterium of Human Gut Bacteria In Vitro.

Jiankuan Li,Jianping Gao,Lina Dong,Yue Liu,Krishnadas Nandakumar

doi:10.1155/2021/9524913

Abstract

Objective To evaluate the prebiotic effects of Codonopsis pilosula polysaccharide (CPP) on human gut bacteria in vitro. Methods Codonopsis Radix was extracted with water at 100°C, and the extract was precipitated by 80% ethanol to obtain CPP. Human fresh fecal samples were collected from three healthy adults and used to ferment CPP. The fermented samples were collected to be analyzed by 16S rRNA sequencing. Results The results showed that CPP exhibited significantly the stimulation on the growth of genus Bifidobacterium of human gut bacteria (Padj < 0.05). Although CPP also exhibited regulative trends on the genera including Acidaminococcus, Bilophila, Dorea, and Eggerthella, no significant differences were observed (Padj > 0.05), which was likely associated with the limited samples (n = 3). Conclusion CPP has the potential to stimulate the growth of Bifidobacterium of the human gut bacteria and to be benefit to human health.

Highlights

Codonopsis Radix, derived from the roots of Codonopsis pilosula (Franch.) Nannf., Codonopsis pilosula (Franch.) Nannf
Zhang et al reported that Codonopsis pilosula polysaccharides attenuated tau hyperphosphorylation and cognitive impairments in adeno-associated virus serotype-induced expression of human full length tau in C57/BL6 mice [2]
E previous study by Jing et al showed that Codonopsis pilosula polysaccharides exhibited the stimulation on the growth of Bifidobacterium, Lactobacillus, and Akkermansia probiotics in dextran sulfate sodium-induced colitis mice [9]. e present study showed that only Bifidobacterium of human gut bacteria was stimulated by CPP during 24 h fermentation

Summary

Objective

To evaluate the prebiotic effects of Codonopsis pilosula polysaccharide (CPP) on human gut bacteria in vitro. Codonopsis Radix was extracted with water at 100°C, and the extract was precipitated by 80% ethanol to obtain CPP. Human fresh fecal samples were collected from three healthy adults and used to ferment CPP. E fermented samples were collected to be analyzed by 16S rRNA sequencing. E results showed that CPP exhibited significantly the stimulation on the growth of genus Bifidobacterium of human gut bacteria (Padj < 0.05). CPP exhibited regulative trends on the genera including Acidaminococcus, Bilophila, Dorea, and Eggerthella, no significant differences were observed (Padj > 0.05), which was likely associated with the limited samples (n 3). CPP has the potential to stimulate the growth of Bifidobacterium of the human gut bacteria and to be benefit to human health

Introduction

Methods

Results and Discussion

Conclusion