Abstract
Synthetic substance P stimulated adenylate cyclase activity in particulate preparations from rat and human brain. The concentration of substance P for half maximal stimulation in rat brain was 1.8 · 10 −7 M. The stimulatory effect of substance P on the rat brain adenylate cyclase activity was 88% compared with 48% by noradrenalin, 163% by prostaglandin E 1 and 184% by prostaglandin E 2. Both the basal and substance P-stimulated adenylate cyclase activity in rat brain were inhibited by concentration of Ca 2+ above 10 −6 M. The chelating agent ethyleneglycol-bis-( β-aminoethylether)- N, N′-tetraacetic acid at a concentration of 0.1 mM reduced the basal adenylate cyclase activity by 64% and eliminated the substance P-stimulated activity. The inhibition by ethyleneglycol-bis-( β-aminoethylether)- N, N′-tetraacetic acid was completely reversed by increasing concentrations of Ca 2+.
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