Abstract
Addition of abscisic acid (ABA) and/or proline stimulated benzyladenine (BA)-induced multiple shoots directly from cotyledon explants (2mm×2mm) of Cucumis sativus in MS medium (Murashige and Skoog, 1962) with 3% sucrose. Stimulation of BA-induced multiple shoots by proline was statistically significant within the concentration range of 5mM-10mM. Although at high levels of 20mM and 30mM, shoots were slightly inhibited. Stimulation of multiple shoots by ABA was optimum at 50μM with higher concentrations of 200μM and 300μM being very inhibitory. Addition of 50μM ABA with 10mM proline stimulated better quality multiple shoots and induction was observed in a maximum percentage (90%) of the explants.
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