Abstract
The blastogenic response to autologous blood lymphocytes to whole-cell suspensions and to homogenates obtained from malignant lymphoma tissue has been investigated. Spleens were obtained from patients in whom laparotomy was performed for staging of malignant lymphoma. Cell suspensions prepared from tumour nodules were treated with mitomycin C and allowed to react with separated autologous blood lymphocytes for 6 days. Lymphocyte stimulation was measured by liquid scintillation counting after exposure to 3H-TdR. Cultures were also prepared in which autologous lymphocytes were treated with spleen tumour homogenate. Control experiments used spleens from staging procedures in which no tumour deposits were present, and normal spleens removed incidentally during other operations. In the controls, the uptake of TdR was never more than twice that of unstimulated lymphocytes. Greater degrees of lymphocyte stimulation were seen in 6 out of 14 patients, using whole tumour cells, and in 7 out of 16 patients, using tumour homogenates. The results indicate an antigenic difference between tumour and host cells, and suggest that lymphocytes can react to a tumour-associated antigen.
Highlights
Summary.-The blastogenic response of autologous blood lymphocytes to whole-cell suspensions and to homogenates obtained from malignant lymphoma tissue has been investigated
MANY human tumours have been shown to have antigenic differences from normal autologous cells (Lewis, 1967; Morton et al, 1968; Helistrom et al, 1971). Immunization to these antigens may occur in vivo (Powles, Balchin and Fairley, 1971) and sensitized lymphocytes may be detected by a blastogenic response on confrontation with whole tumour cells or tumour extracts (Mavligit et al, 1973; Vanky et al, 1974), using methods similar to those used in a one-way mixed lymphocyte reaction (Bach and Voynow, 1966)
Patients.-Spleens were obtained from patients in whom laparotomy was performed in order to stage the anatomical extent of malignant lymphoma or to provide the initial diagnosis, from patients in whom the spleen was removed for other non-neoplastic haematological diseases, and from patients in whom a histologically normal spleen was removed during the course of another operation
Summary
Summary.-The blastogenic response of autologous blood lymphocytes to whole-cell suspensions and to homogenates obtained from malignant lymphoma tissue has been investigated. We have investigated the response of autologous lymphocytes to lymphoma cell suspensions and to subcellular preparations of lymphoma tissue. Heterologous responses, in which spleen homogenates were used to stimulate lymphocytes from normal individuals were tested.
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