Abstract
Arachidonic acid (AA) regulates many aspects of vascular smooth muscle behaviour, but the mechanisms linking receptors to AA release are unclear. In A7r5 vascular smooth muscle cells pre-labelled with 3H-AA, vasopressin caused a concentration-dependent stimulation of 3H-AA release that required phospholipase C and an increase in cytosolic [Ca 2+]. Ca 2+ release from intracellular stores and Ca 2+ entry via L-type channels or the capacitative Ca 2+ entry pathway were each effective to varying degrees. Selective inhibitors of PLA 2 inhibited the 3H-AA release evoked by vasopressin, though not the underlying Ca 2+ signals, and established that cPLA 2 mediates the release of AA. We conclude that in A7r5 cells vasopressin stimulates AA release via a Ca 2+-dependent activation of cPLA 2.
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