Abstract
Intravitreal injection of ouabain was used to induce unilateral hypotony and to study the relationship of adenylate cyclase (AC) and sodium-potassium activated adenosine triphosphatase (NaK-ATPase) both of which are involved in the production of aqueous humour. After preliminary experiments, days 3 and 5 were chosen as representative times when IOP was maximally reduced and stable following ouabain injection. NaK-ATPase and adenylate cyclase activities were measured biochemically in the same homogenates of isolated ciliary processes (CP). Biochemical measurements showed that 46% of NaK-ATPase activity was inhibited after 3 days, and about 78% of NaK-ATPase was inhibited 5 days after ouabain injection. At the beginning of NaK-ATPase inhibition there was a significant stimulation of adenylate cyclase activity of the CP. The highest activities were seen 2 and 3 days after ouabain injection. The suggestion is made that these 2 enzymes are interdependent.
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