Stimulation of adenosine 3′,5′-monophosphate formation in mononuclear leukocytes by toad venoms

Abstract

The content of adenosine 3′,5′-monophosphate in human mononuclear leukocytes was enhanced 3–5-times by venoms obtained from African toad ( Bufo africanus), American toad ( Bufo americanus), Colorado river toad ( Bufo arenarum) and Marine toad ( Bufo marinus) at 25 μg/ml for 5 min of incubation at 37°C. The maximum stimulation was observed after 1–5 min of incubation. The half-maximal stimulation was observed at 0.1 μg/ml venom obtained from Colorado river toad ( Bufo arenarum). The increased content of adenosine 3′,5′-monophosphate in the mononuclear leukocytes persisted without significant change for at least 30 min of incubation at 37°C.