Abstract
Dendritic spines are tiny protrusions along dendrites that receive excitatory synaptic inputs and compartmentalize postsynaptic responses in the mature brain. It is known that change in spine morphology is associated with brain functions such as learning and memory. α2A-Adrenoceptors (α2A-ARs) are highly expressed in cortical neurons and play important roles in neuronal differentiation, growth and neurotrophy. However, little is known about the role of α2A-ARs in the maturation of dendritic spines. Here, we report that stimulation of α2A-ARs promotes the maturation of dendritic spines in cultured neurons of the medial prefrontal cortex of rodents. Our results show that, stimulation of α2A-ARs by guanfacine induced significantly more stubby or mushroom spines in cultured mPFC neurons, with an enlargement of the spine head size. In parallel, the expression of PSD95 (a postsynaptic protein) in guanfacine-treated neurons was enhanced, while that of synapsin (a pre-synaptic protein) kept unchanged. These effects of guanfacine were blocked by co-administered yohimbine, a non-selective α2-AR antagonist. The present results implicate a prominent role of α2A-ARs in regulating the maturation of dendritic spines in the mPFC.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have