Stimulated Raman Scattering Microscopy for Label-Free Chemical Imaging

X Sunney Xie,Christian W Freudiger,Brian G Saar,Wei Min

doi:10.1364/opn.20.12.000030

Abstract

Coherent anti-Stokes Raman techniques are increasing the utility of Raman scattering for chemical and biological diagnostics.

Highlights

We recently developed a new multiphoton imaging technique, stimulated Raman scattering (SRS) microscopy, which allows chemical imaging of biological samples without the need for staining or fluorescent labeling.[1]
Such label-free imaging is desirable in biomedical research, because labeling often perturbs the function of small metabolite and drug molecules and may be too toxic to use in vivo in medical diagnostics
Stimulated Raman scattering microscopy had been reported by Ploetz et al.,[2] the high peak power lasers and low sensitivity were incompatible with biological applications

Summary

Introduction

We recently developed a new multiphoton imaging technique, stimulated Raman scattering (SRS) microscopy, which allows chemical imaging of biological samples without the need for staining or fluorescent labeling.[1]. We recently developed a new multiphoton imaging technique, stimulated Raman scattering (SRS) microscopy, which allows chemical imaging of biological samples without the need for staining or fluorescent labeling.[1] Such label-free imaging is desirable in biomedical research, because labeling often perturbs the function of small metabolite and drug molecules and may be too toxic to use in vivo in medical diagnostics. The contrast of the technique originates from molecular vibrational frequencies that are intrinsic to specific chemical compounds.

Results

Conclusion