Sterols ofArtemia salina

Abstract

In order to study the sterols, a mixture of 10.35 g of dewatered shrimps and 41.20 g of a 10% solution of potassium hydroxide in ethanol was boiled under reflux for 1 h and was extracted with diethyl ether (3 × i00 ml). The combined ethereal extracts were washed with saturated aqueous sodium chloride solution and dried with anhydrous sodium sulfate. After the solvent had been distilled off, 0.240 g of unsaponifiable residue was obtained. This 0.240 g of unsaponifiable residue was chromatographed on a column (700 × i0 mm) of silica gel (KSK, grain size 0.140-0.315 mm) to give 0.096 g of a white solid product.