Abstract
Human serum albumin and phospholipids can interact to cause a synergistic sterol release from mammalian cells in tissue culture. In the presence of the complexes formed between albumin and saturated phosphatidylcholine, release was twice as great as that which occurred with the unsaturated phospholipid complexes. Within the saturated series, sterol release increased with chain length until the number of carbon atoms in the acyl group was 18, after which sterol release decreased. In the unsaturated series, sterol release decreased as the number of double bonds increased. Branching of the acyl chain, or analogues with the polar group in the sn-2 position reduced sterol efflux. In the presence of human serum albumin, maximal sterol efflux occurred with phospholipids having two adjacent acyl chains and zero net charge; sterol release decreased as net charge increased.
Highlights
Human serum albuminand phospholipids can inter- examination of an homologousphospholipid series in the act to causea synergistic sterol release from mamma- presence and absence of acceptor proteins has not been prelian cells in tissue culture
The observed lytic effect of dicapryl and dilauryl phosphatidylcholines is in agreement with Reman et al (19)who showed the same effect on red blood cells by phosphatidylcholines having an acyl chain length of 10 or 12 carbon atoms
There were small significant differences in sterol release induced by the various phosphatidylcholines individually (TablesI1 and IV), thmeagnitude of the response
Summary
Human serum albuminand phospholipids can inter- examination of an homologousphospholipid series in the act to causea synergistic sterol release from mamma- presence and absence of acceptor proteins has not been prelian cells in tissue culture. Sterol release increased with chain length until the number of carbon. In the presence of human serum albumin, maximal sterol efflux occurred with phospholipidshaving through a 0.22 p Millipore filter (Millipore Corp., Bedford, MA). The stock cultures were antibiotic-free, thatan albumin-dipalmitoyl phosphatidylcholine complex but experimental cultures contained penicillin (200 units/ml)and causes synergistic sterol release from human and animal cells in tissue culture. Serum albumin induces a 10-fold in- 3-phosphocholine and l-palmitoyl-2-oleoyl-sn-glycero-3-phosphochocrease in cholesterol transport between liposomes and human skin fibroblasts (3),binds phospholipid under cell-free conditions (4, 5), and increases phospholipid efflux from cells (6). Recent reports that albumin influences lecithin-cholesterol line from Supelco, Bellefonte, PA; l-stearoyl-2-oleoyl-sn-glycero-3phosphocholine from Applied Science Laboratories, and the remaining phospholipids from Calbiochem-Behring
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