Sterol Composition of the Arthrospores and Mycelium of the Fungus Mucor hiemalis

Abstract

Sterol composition of the arthrospores and mycelium of the fungus Mucor hiemalis 1156 was studied by the method of chromatography–mass spectrometry. Along with ergosterol, the major sterol of the culture studied, ten minor sterols were identified, which were either precursors or products of ergosterol degradation. The content of individual sterols differed substantially in arthrospores and mycelium, which represent different stages of ontogenetic development of the fungus. In arthrospores, the content of ergosterol was lower than in mycelium (55.9 and 78.0%, respectively). Among the precursors of ergosterol, methylated sterols predominated in arthrospores (24.1% versus 11.6% in mycelium). Eburicol and 4,4-dimethylfecosterol were the major methylated sterols of arthrospores (10.6 and 8.1%, respectively). In addition, two uncommon and extremely rare sterols, 1-dihydro-dehydroneoergosterol and dehydroneoergosterol, were identified (for the first time in M. hiemalis). These substances, containing a complex system of conjugated double bonds in their A and B rings, are the products of ergosterol degradation. The data on sterol composition are discussed in terms of their morphogenetic implication.