Abstract
AbstractThe effects of the fungicides, flutriafol, triadimenol and an experimental compound (2,2‐dimethyl‐3‐(2‐chlorobenzyl)‐4‐(1,2,4‐triazol‐1‐yl)‐butan‐3‐ol), on conidial viability and lipid composition, have been examined in strains of barley and wheat powdery mildew (Erysiphe graminis DC.) sensitive or resistant to DMI fungicides. In the absence of fungicides, these strains had the same sterol composition, regardless of their sensitivity to DMIs. Following treatment with the compounds, conidia contained eburicol and obtusifoliol, indicating inhibition of the 14α‐demethylase. There was also an accumulation of episterol, suggesting that introduction of the 5,6‐double bond was also inhibited. The changes in sterol composition induced by the fungicides were small. The fatty acid composition of sensitive and resistant strains was also determined. Triadimenol treatment caused an increase in the saturation of C22 and C24 acids in all strains examined, but changes were not correlated with the biological effects of the fungicide. No single mechanism of resistance emerged from the study.
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