Abstract
ABSTRACT A pool of 2.6 1 urine was collected from normal male newborns for the first 5 days of life. After Amberlite XAD-2 column chromatography, enzyme hydrolysis and extraction, the steroid residue was fractionated by celite column chromatography and each fraction purified by multiple thin-layer chromatography. Identification and quantitation was achieved by gas-liquid chromatography and gas-liquid chromatography-mass spectrometry. Progesterone 4.6 μg/l, pregnanediol 729 μg/1, 16α-hydroxyprogesterone 713 μg/1, 16α-hydroxypregnenolone 14,000 μ/1, 16α-hydroxydehydroepiandrosterone 2,350 μg/l, 16-dehydroprogesterone 5.3 μg/1, 16-dehydropregnenolone 174 μg/1. The identification of 16-dehydropregnanolone is indicated. Comparison with findings in cord plasma and amniotic fluid are done and the role of Δ16-C21 steroids in newborn urine is discussed.
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