Abstract

The apparatus and method for centrifugally accelerated partition chromatography of steroids is described. It is shown that the centrifugal velocity must be relatively low for separations to occur. The other factors studied were the mode of sample application and the stationary phase content of the paper. Using the described procedure, separations occur in 10-15 min. This makes it particularly suited to analyses which require short development times such as in chemical or enzymic kinetic studies or to determine the homogeneity of fractions in column chromatography.

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