Abstract
Two new furostanol saponins 1–2 and a new spirostanol saponin 3 were isolated together with two known furostanol saponins 4–5 from the roots and rhizomes of Tupistra chinensis. Their structures were characterized as 1β,2β,3β,4β,5β,26-hexahydroxyfurost-20(22),25(27)-dien-5,26-O-β-d-glucopyranoside (1), 1β,2β,3β,4β,5β,6β,7α,23ξ,26-nona-hydroxyfurost-20(22),25(27)-dien-26-O-β-d-glucopyranoside (2), (20S,22R)-spirost-25 (27)-en-1β,3β,5β-trihydroxy-1-O-β-d-xyloside (3), tupisteroide B (4) and 5β-furost-Δ25(27)-en-1β,2β,3β,4β,5β,7α,22ξ,26-octahydroxy-6-one-26-O-β-d-glucopyranoside (5), respectively, by extensive use of spectroscopic techniques and chemical evidence. Additionally, the in vitro cytotoxic activity of 1–4 was evaluated on human A549 and H1299 tumor cell lines, and compound 3 exhibited cytotoxicity against A549 cells (IC50 86.63 ± 2.33 μmol·L−1) and H1299 cells (IC50 88.21 ± 1.34 μmol·L−1).
Highlights
Tupistra chinensis Baker., a species in the Tupistra genus of the Liliaceae family, is used as an endemic herbal medicine, known as “Kai-Kou-Jian”, in the Qinba Mountains of Shaanxi Province in China [1]
The roots and rhizomes of T. chinensis are commonly used as folk medicine to treat throat irritation, rheumatic diseases and snake-bites [2,3]
The cytotoxic activity of 1–4 was evaluated on human A549 and H1299 tumor cells
Summary
Tupistra chinensis Baker., a species in the Tupistra genus of the Liliaceae family, is used as an endemic herbal medicine, known as “Kai-Kou-Jian”, in the Qinba Mountains of Shaanxi Province in China [1]. The roots and rhizomes of T. chinensis are commonly used as folk medicine to treat throat irritation, rheumatic diseases and snake-bites [2,3]. As part of our research project to find more diverse bioactive leading compounds from the medicinal herbs of the Qinba Mountains [8,9,10,11], the chemical constituents and pharmacological studies of T. chinensis were investigated, and two new furostanol saponins, 1β,2β,3β,4β,5β,26-hexahydroxyfurost-20(22),25(27)-dien-5,26-O-β-D-glucopyranoside (1), 1β,2β,3β,4β,5β,6β,7α,23ξ,26-nonahydroxyfurost-20(22),25(27)-dien-26-O-β-D-glucopyranoside (2), and a new spirostanol saponin (20S,22R)-spirost-25(27)-en-1β,3β,5β-trihydroxy-1-O-β-D-xyloside (3). Were obtained from the roots and rhizomes of T. chinensis together with the two known compounds tupisteroide B (4) and 5β-furost-Δ25(27)-en-1β,2β,3β,4β,5β,7α,22ξ,26-octahydroxy-6-one-26-O-β-Dglucopyranoside (5) (Figure 1). The cytotoxic activity of 1–4 was evaluated on human A549 and H1299 tumor cells
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