Abstract
Ovarian follicles were isolated from virgin New Zealand White rabbits and incubated in medium containing Hanks balanced salt solution. Medium 199 and normal rabbit serum. Medium was changed every 15 min and stored at — 15°C until assayed for progestin and androgen by established radioimmunoassay procedures. At fixed time intervals after the start of the incubations various cyclic nucleotides, theophylline or sodium fluoride were added. Of the nucleotides tested only cyclic AMP, dibutyryl cyclic AMP and cyclic CMP influenced steroid secretion; androgen production increased from negligible levels to about 10 ng/ml and progestin from 0.5 mg/ml to approximately 3 ng/ml. Sodium fluoride had no effect at any of the doses tested. Adenosine 5'-monophosphate also stimulated steroid secretion. These data suggest that in the intact rabbit follicle cyclic AMP probably mediates the response of the follicles to tropic hormone stimulation.
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