Abstract
High-performance liquid chromatography analysis of extracts of Arctic charr (Salvelinus alpinus) eggs revealed the presence of several steroids, predominantly progestogens together with testosterone. Yolk sac embryos were incubated with tritiated progesterone ([3H]P4) or 17-hydroxyprogesterone ([3H]17OHP) to examine the ability of the embryos to metabolize progestogensin vitro;both progestogen precursors were converted to various free and conjugated steroids metabolites (sulfates and glucuronides). [3H]P4was completely metabolized to form steroids that coeluted with standard 11-oxygenated androgens, corticosteroids, progestogens, and some other unidentified metabolites. This report also describes the biosynthesis of 17,20β-dihydroxy-4-pregnen-3-one and 17,20β-dihydroxy-4-pregnen-3-one 20-sulfate by embryos of Arctic charr that were incubated with 17OHP.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.