Steroid hormones, ectoparasites, and color: Sex, species, and seasonal differences in Sceloporus lizards

Abstract

Testosterone, in addition to promoting the expression of sexual ornaments can negatively affect immune function, leaving individuals more susceptible to parasites (immunocompetence handicap hypothesis). Immunosuppressive effects of testosterone also can occur indirectly, through increased glucocorticoid hormones (corticosterone, cortisol). Therefore, the expression of sexual ornaments and the ability to respond to parasites and diseases may be influenced by the interaction between testosterone and corticosterone. In this study we examined correlations of both testosterone and corticosterone with ectoparasite load and with expression of colorful sexual ornaments (patches on belly and throat) in three species of Sceloporus lizards (S. grammicus, S. megalepidurus, S. torquatus). In addition, we evaluated contributions of sex, body condition, and reproductive season. We expected that individuals with higher testosterone and lower corticosterone levels would have more colorful ornaments than individuals with higher corticosterone levels. In addition, if testosterone has negative effects on immune function but only at higher corticosterone levels, individuals with higher levels of testosterone and corticosterone should have higher ectoparasite loads. Contrary to these expectations, we did not detect an interaction between testosterone and corticosterone statistically affecting either ectoparasite load or the expression of colorful ornaments. Further, we did not find a positive association of either testosterone or corticosterone on ectoparasite loads in any of the three study species. Only in S. grammicus males was a hormone statistically associated with ectoparasite load, but it was a negative association with testosterone. The relationships between both hormones and different aspects of the colorful patches (brightness and chroma) varied drastically among the three species as well as between sexes. Hence, even among congeneric species, we did not observe consistent patterns among color, steroid hormones and ectoparasites. Different associations between these variables may reflect different physiological strategies for the production of colorful signals and immune defense.