Steroid hormone receptor localization in the nuclear matrix: Interaction with acceptor sites

Abstract

The nuclear matrix is a conceptually attractive candidate for the site in the nucleus where steroid hormone-receptor complexes might interact to modulate DNA structure and function. We have demonstrated that in sex steroid target tissues a major proportion (50–100%) of the high affinity and steroid-specific receptors that become associated with the nucleus following hormonal stimulation are localized in the nuclear matrix. Direct cell-free binding assays confirm that this localization is due to the presence of specific acceptor sites in the matrix to which steroid-receptor complexes bind with high affinity and tissue specificity, and is not the result of spurious binding. The nuclear matrix appears to be a major site of hormone receptor binding in the nucleus, and this situation is consistent with the known ability of steroid hormones to stimulate gene transcription, a process which also appears to occur in association with the nuclear matrix.