Abstract
Daun dewa (Gynura pseudochina Lour DC) is a one of popular traditional medicine to treat various diseases. This research was conducted to isolate chemical compounds from daun dewa leaves using various chromatographic techniques. A steroid mixture namely b-sitosterol (1a) and stigmasterol (1b) were isolated for the first time from the methanol extract of daun dewa. The structures were determined base on spectral evidence including IR, NMR 1D and NMR 2D.
Highlights
Gynura pseudochina (Lour) DC which locally name in Indonesia known as daun dewa is belongs to the Asteraceae family
A solid white compound (165 mg) with m.p 123-125 oC was isolated from the methanol extract of G. pseudochina after separated by several chromatographic techniques
The nuclear overhouser effect spectroscopy (NOESY) spectra showed correlation between methine signal at H 2.04 ppm (H-20) with a methyl signal at H 0.69 ppm (H-18) and an olefinic proton signal at 5.01 ppm (H-23), indicated they were in the same side
Summary
Gynura pseudochina (Lour) DC which locally name in Indonesia known as daun dewa is belongs to the Asteraceae family. As a part of our research on phytochemistry from G. pseudochina, two steroid compound namely -sitosterol (1a) and stigmasterol (1b) have been isolated from methanol extract of this plant. The steroid was isolated as a mixture compound.
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