Abstract

Sterols of whole adult Colorado potato beetles, Leptinotarsa decemlineata Say, as well as of their larvae and pupae have been studied. Using chromatographic and mass spectrographic methods (molecular mass spectrography employing negative ionization) the same sterols and triterpenoids could be detected as in leaves of the food plant of this insect, Solanum tuberosum L., but in quite different ratios. The principal sterols of the insect are cholesterol and β-sitosterol. The other ones, presumably campesterol and stigmasterol, as well as the 4α-methyl sterols and the triterpen cycloartenol occur in much lower amounts than in the potato plant. Larvae were shown to contain paraffin hydrocarbons of surprisingly high molecular weights, particularly tetrapentacontane (C 54 H 110 ), pentapentacontane (C 55 H 112 ), and heptapentacontane (C 57 H 116 ).

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