Abstract
This paper reports on the effects of organic ingredients in facilitating direct shoot regeneration from nodal explants of Hibiscus rosa-sinensis L. This paper also compares the sterilization conditions for 3 types of explants (node, internode, and shoot tip) harvested from an open field. The optimized sterilization conditions for the explants were 40% Clorox- 20 min exposure, 10% Clorox-15 min exposure, and 5% Clorox-40 min exposure for the node, internode and shoot tip, respectively. In the direct shoot regeneration using the nodal explants, we found MS medium containing 40 g/L sucrose, 0.3% (w/v) activated charcoal, and supplementations with myo-inositol, thiamine and nicotinic acid were suitable. The in vitro shoot survival rate was 30% with a mean leaf numbers of 2.68 produced, and a mean leaf length of 1.71 cm achieved after 5 weeks of culture on the modified medium.
Highlights
Hibiscus rosa-sinensis L., commonly known as “Chinese Hibiscus”, is an evergreen perennial plant that grows in tropical and sub-tropical regions
This paper reports on the effects of organic ingredients in facilitating direct shoot regeneration from nodal explants of Hibiscus rosa-sinensis L
For media used in the sterilization process, 20 g/L of sucrose was added to half strength MS (1/2 MS) basal medium while for subsequent direct shoot induction, different sucrose concentrations (10, 20, 30, 40, 50 g/L), activated charcoal [0.1, 0.2, 0.3, 0.4 and 0.5% (w/v)] and different combinations of organic ingredients [myoinositol + thiamine.HCl, myoinositol + thiamine.HCl + nicotinic acid, myoinositol + thiamine.HCl + pyridoxine.HCl, myoinositol + thiamine
Summary
Hibiscus rosa-sinensis L., commonly known as “Chinese Hibiscus”, is an evergreen perennial plant that grows in tropical and sub-tropical regions. It has a charismatic bright red, five-petal, and single layer flower, and the leaves are alternate along the branch. Vegetative propagation can shorten the length of the juvenile period and allows genotypes combination [11] Such traditional asexual propagation cannot circumvent the very fundamental problems of long growing period, large growing space, small plant quantity, and inefficiency. After approximately 100 years of development, plant tissue culture technique provides an alternative to solving those problems. The plant tissues can be the internodes, nodes, shoot tips (stems), root tips, calli, leaves, seed embryos, or anthers
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