Abstract
Introduction: The need of effective sterilization method and their monitoring is necessary. Biological indicators are specific microorganisms with high resistance toward particular sterilization methods. Their processes include steam autoclave, dry heat sterilizer, ethylene oxide sterilizer. This article has considered various methods to monitor the effectiveness of different sterilization methods used in orthodontics. Materials and Methods: The parameters for comparison were the control and experimental instruments utilized in orthodontic treatment. The efficacy of sterilization was evaluated by comparison of bacterial growth obtained in monitoring by biological indicators and swab test method. Results: No spore growth was found when sterilization process was evaluated by biological indicators in comparison to swab test where spore growth was present. Instruments dipped in Bioclenz-G solution for 10 min showed spore growth, but no spore growth was seen in 10 h cycle. Discussion: The result of the study verifies the established effectiveness of biological indicators over conventional swab test method in monitoring various sterilization processes used in orthodontics. Bioclenz-G solution can be used as an effective cold sterilization method for sterilization. Conclusion: For evaluating the effectiveness of sterilization, biological indicators preclude the drawbacks of incomplete verification of destruction of all vegetation and inordinate delay in procurement of results as is the case with chemical indicators and lab culture, respectively.
Highlights
The need of effective sterilization method and their monitoring is necessary
The baseline parameters for comparative results of all experimental and control group tested by conventional swab test method and biological indicator method reveal that by conventional swab test method in steam sterilization, no spore growth was found in medium load in all the 15 groups tested in experimental group
The type of sterilization can depend upon a variety of factors including critical, noncritical instruments, type of instruments that is, hinged, nonhinged, etc., The current study evaluated the following sterilization processes–steam autoclave, hot air oven, ethylene oxide sterilizer, and Bioclenz‐G solution
Summary
Biological indicators are specific microorganisms with high resistance toward particular sterilization methods. Their processes include steam autoclave, dry heat sterilizer, ethylene oxide sterilizer. This article has considered various methods to monitor the effectiveness of different sterilization methods used in orthodontics. The efficacy of sterilization was evaluated by comparison of bacterial growth obtained in monitoring by biological indicators and swab test method. Results: No spore growth was found when sterilization process was evaluated by biological indicators in comparison to swab test where spore growth was present. Discussion: The result of the study verifies the established effectiveness of biological indicators over conventional swab test method in monitoring various sterilization processes used in orthodontics. Sterilization, removes all forms of microorganisms including viruses, bacteria, fungi, and spores
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