Abstract
The stereospecificity of hydrogen transfer in the synthesis of saccharopine from α-ketoglutarate and l-lysine catalyzed by saccharopine dehydrogenase ( N 5- (1,3-dicarboxypropyl)-L-lysine : NAD oxidoreductase (L-lysine-forming) EC 1.5.1.7) was examined by using [4A- 3H]- and [4B- 3H] NADH. The enzyme showed the A-stereospecificity. The NMR analysis of the saccharopine prepared with [4A- 2H]NADH revealed that the label was incorporated into the C-2 of the glutaryl moiety.
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