Abstract
We determined the positional fatty acid distribution of chufa tuber at stereospecific positions; the sn-1, sn-2, and sn-3 positions in the triacylglycerols of chufa tuber oil. Chufa tubers were washed with water, and oven dried to a moisture content of 20%. The total lipid (TL) was extracted in a Soxhlet apparatus using a mixture of chloroform and methanol at 40 � C. The total lipid extracted was dissolved in chloroform and separated into neutral lipids (NL), glycolipids (GL) and phospholipids (PL) using silicic acid column chromatography [7]. Stereospecific analysis of chufa oil was carried out using pancreatic lipase, phospholipase A2, TLC and GC [8]. The fatty acid composition was determined using gas chromatography [9]. The fatty composition at the sn-3 position was calculated based on the equation, 3 · [TG]‐[sn-1 + sn-2 position].
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