Abstract
To elevate the concentration of racemic styrene oxide (rac-SO), its asymmetric hydrolysis in organic/aqueous biphasic system was researched. Firstly, n-octane/phosphate buffer reaction system was constructed, where PvEH2 retained 75.9% activity at 25 °C for 8 h. Secondly, the optimal volume ratio of two phases and weight ratio of rac-SO to E. coli/pveh2 were 4:6 and 0.8. Then, the maximum allowable rac-SO concentration was assayed as 200 mM, 10-fold that in aqueous phase. Finally, the hydrolysis of rac-SO was performed, affording (R)-SO with over 99.5% ees and 47.8% yield, and (R)-phenyl-1,2-ethanediol with 93.6% eep and 50.4% yield in 12 h.
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