Stereological analysis of hippocampus in rat treated with chemotherapeutic agent oxaliplatin.

Abstract

Oxaliplatin (OX) has been widely used for treatment of colorectal and other cancers. Adverse effect of OX and other anticancer agents on cognition have been reported, but studies on the effects of chemotherapy on brain structure are scarce. This study describes the morphometrical features of the hippocampus structures in rat following OX treatment using design-based stereological methods. Ten male Wistar rats were randomised into two groups. The rats from OX group received 2.4 mg/kg OX in vehicle for 5 consecutive days every week for 2 weeks intraperitoneally. Controls received vehicle only. Cavalieri's method and the optical fractionator method were used for volume and neuron estimation, respectively. Cavalieri's method was used to estimate volume and showed that the volume of the hippocampus was significantly decreased in OX group (31.84 ± ± 1.24 mm3) compared with the vehicle control group (36.95 ± 3.48 mm3). The optical fractionator method was used to estimate neuron number and showed that the number of neurons in dentate gyrus, cornu ammonis 1 and 3 in OX group (8.147 ± 2.84 × 105, 4.257 ± 0.59 × 105 and 2.133 ± 0.22 × 105, respectively) did not differ from those of vehicle control group (7.36 ± 1.42 × 105, 3.521 ± ± 0.54 × 105 and 1.989 ± 0.46 × 105, respectively). These findings suggested that OX treatment induces loss of hippocampal volume without neuronal loss which might help to clarify the mechanism by which OX affects cognition and to improve preventive treatment strategies.