Abstract
The stereochemistry of hydrogen transfer between pyridine nucleotide and some intermediates of cholesterol catabolism was studied. 1. 1. The hydrogen transferred from NADH to 3α,7α,12α-trihydroxy-5β-cholestan-26-al, 3-oxo-5β-cholan-24-oic acid and to acetaldehyde catalyzed by rat liver alcohol:NAD oxidoreductase (EC 1.1.1.1) was the 4A-hydrogen. 2. 2. The hydrogen transferred from 3α,7α,12α-trihydroxy-5β-cholestan-26-al and from acetaldehyde to NAD + catalyzed by horse liver aldehyde dehydrogenase (EC 1.2.1.3) was the 4A-hydrogen.
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