Abstract
Treatment of 32P-labeled cauliflower mosaic virus (CaMV) with 0.02% pronase for 2–5 min at 37° degraded the viral protein shell, producing particles of about 100 S and finally nucleic acid free from protein. Incubation of 35S-labeled CaMV under the above conditions resulted in the degradation of virus particles to about 100 S and finally protein remaining in the top of the gradient. The slower-sedimenting particles show the regular loss of protein moiety in a stepwise fashion with the time of incubation in pronase, suggesting that CaMV particles consist of some layers of protein shells. Tobacco mosaic virus (TMV), however, is not degraded by the same treatment with pronase as CaMV. Pronase digestion is rather specific for some viruses. The structure of CaMV is discussed in comparison with that of polyoma virus and simian virus 40.
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