Stenotrophomonas nematodicola sp. nov., isolated from Caenorhabditis elegans.

Abstract

A Gram stain-negative, coccoid rod-shaped, motile by gliding, facultatively aerobic bacterium, designated W5, was isolated from Caenorhabditis elegans samples in BaotianmanNatural Reserve (33° 27' 47'' N; 111° 48' 32'' E), Nanyang, China. The isolate was characterized taxonomically using a polyphasic approach. The 16S rRNA gene of strain W5 exhibited 98.1-99.7% similarity to the 16S rRNA genes of members of the genus Stenotrophomonas, and < 98.0% similarities to those of other bacterial species in the family Lysobacteraceae. The most closely related strains were Stenotrophomonas rhizophila JCM 13333T (99.7%) and Stenotrophomonasbentonitica DSM 103927T (99.2%). The predominant respiratory quinone of the isolate is Q-8. The major fatty acids are iso-C15:0 (38.2%) and antesio-C15:0 (16.6%). The draft genome of strain W5 had a length of 4,402,751bp and a DNA G + C content of 67.3mol%. The ANI values between the draft genomes of strain W5 and its closest phylogenetic neighbors S. rhizophila JCM 1333T and S. bentonitica DSM 103927T were 84.7% and 85.0%, respectively. The DDH value between W5 and S. rhizophila JCM 13333T was 30.8%, which was the highest DDH level. We propose that strain W5 represents a novel bacterial species with the name Stenotrophomonas nematodicola sp. nov. and W5 as the type strain. The type strain is W5 (= CPCC 101271T = CGMCC 19401T = KCTC XXXT).