Abstract

A Gram-stain negative, rod-shaped, aerobic bacterial strain, BII-R7T, was isolated during a study targeting the culture-dependent microbial diversity occurring in bentonite formations from southern Spain. Comparative 16S rRNA gene sequence analysis showed that BII-R7T represented a member of the genus Stenotrophomonas (class Gammaproteobacteria), and was related most closely to Stenotrophomonas rhizophila e-p10T (99.2 % sequence similarity), followed by Stenotrophomonas pavanii ICB 89T (98.5 %), Stenotrophomonas maltophilia IAM 12423T, Stenotrophomonas chelatiphaga LPM-5T and Stenotrophomonas tumulicola T5916-2-1bT (all 98.3 %). Pairwise sequence similarities to all other type strains of species of the genus Stenotrophomonas were below 98 %. Genome-based calculations (orthologous average nucleotide identity, original average nucleotide identity, genome-to-genome distance and DNA G+C percentage) indicated clearly that the isolate represents a novel species within this genus. Different phenotypic analyses, such as the detection of a quinone system composed of the major compound ubiquinone Q-8 and a fatty acid profile with iso-C15 : 0 and anteiso-C15 : 0 as major components, supported this finding at the same time as contributing to a comprehensive characterization of BII-R7T. Based on this polyphasic approach comprising phenotypic and genotypic/molecular characterization, BII-R7T can be differentiated clearly from its phylogenetic neighbours, establishing a novel species for which the name Stenotrophomonas bentonitica sp. nov. is proposed with BII-R7T as the type strain (=LMG 29893T=CECT 9180T=DSM 103927T).

Highlights

  • A Gram-stain negative, rod-shaped, aerobic bacterial strain, BII-R7T, was isolated during a study targeting the culturedependent microbial diversity occurring in bentonite formations from southern Spain

  • This genus was proposed when the species Xanthomonas maltophilia was reclassified as Stenotrophomonas maltophilia [12], and subsequently accommodated in the class Gammaproteobacteria [13]

  • At the time of writing, the genus Stenotrophomonas comprises 13 species with validly published names isolated from a large range of natural and artificial environments and geographical regions including S. maltophilia [12], Stenotrophomonas nitritireducens [14], Author affiliations: 1Departamento de Microbiología, Campus de Fuentenueva, Universidad de Granada, 18071 Granada, Spain; 2Departamento de Genetica, Campus de Fuentenueva, Universidad de Granada, 18071 Granada, Spain; 3Institut für Angewandte Mikrobiologie, Justus-Liebig-Universita€t Giessen, D-35392 Giessen, Germany; 4Institut für Mikrobiologie, Veterin€armedizinische Universita€t Wien, A-1210 Wien, Austria. *Correspondence: Ivan Sanchez-Castro, sanchezcastro@ugr.es Keywords: Stenotrophomonas; BII-R7T; bentonite; ANI value

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Summary

TAXONOMIC DESCRIPTION

Sanchez-Castro et al, Int J Syst Evol Microbiol 2017;67:2779–2786 DOI 10.1099/ijsem.0.002016 Ivan Sanchez-Castro,1,* Miguel Angel Ruiz-Fresneda,1 Mohammed Bakkali,2 Peter Ka€mpfer,3 Stefanie P. Glaeser,3 Hans Jürgen Busse,4 Margarita López-Fernandez,1† Pablo Martínez-Rodríguez1 and Mohamed Larbi Merroun1

Original ANI
Fatty acid
Findings
DESCRIPTION OF STENOTROPHOMONAS
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