Abstract
Mannans are hemicellulosic polysaccharides and are present in cell walls of all land plants. Mannan polysaccharides are synthesized by two enzymes, mannan synthase (ManS) for backbone (mannan or glucomannan) synthesis and galactomannan galactosyl transferase for side-chain (galactosyl) addition. Here, a method for ManS activity assay using microsomes freshly isolated from Arabidopsis stems is described. This method can be applied to isolation of microsomes from any tissues of Arabidopsis or any other plants., 甘露聚糖是半纤维素多糖，并且存在于所有陆地植物的细胞壁中。 甘露聚糖由两种酶合成：用于主链（甘露聚糖或葡甘露聚糖）合成的甘露聚糖合酶（ManS）和用于侧链（半乳糖基）加成的半乳甘露聚糖半乳糖基转移酶。 这里，描述了使用新鲜分离自拟南芥茎的微粒体进行ManS活性测定的方法。 该方法可以应用于从拟南芥或任何其它植物的任何组织分离微粒体。
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