Abstract
BackgroundAlbizia richardiana belongs Fabaceae family which different parts like fruits, flowers, barks, and roots are used medicinally. The study reports the in vitro anti-inflammatory, thrombolytic, cytotoxicity and antimicrobial activity of methanolic extract of A. richardiana stem and its different fractions.MethodThe methanolic extract of A. richardiana stem (MEAR) extracted with n-hexane (HXFAR), carbon tetrachloride (CTFAR), chloroform (CFAR), and aqueous (AQFAR) and subjected for DPPH scavenging activity and total phenol content (TPC). The cytotoxic activity evaluated by brine shrimp lethality bioassay, while the disk diffusion method used for the antimicrobial study. The anti-inflammatory and thrombolytic activities of the extracts evaluated by the hypotonic solution induced hemolysis, heat-induced hemolysis and human blood clot lysis, respectively.ResultsAll the extracts exhibited excellent antioxidant activity in the DPPH scavenging assay and maximum total phenol content observed by HXFAR. Secondly, the extract showed a moderate LC50 value in brine shrimp lethality bioassay, where the CTFAR extract exhibited potential antimicrobial activities against sixteen different microorganisms. In anti-inflammatory, all the extract exhibited a significant (P < 0.0001) protection against lysis of human erythrocyte membrane induced by heat and hypotonic solution, as compared to the standard acetyl salicylic acid. An extremely significant (P < 0.0001) clot lysis was found in MEAR (16.66%) while the standard drug streptokinase (70.94%).ConclusionAll the fractions revealed the significant free radical scavenging activity. Moreover, CTFAR showed wide spectrum of antimicrobial activity. Thus, the results of the present study provided scientific evidence for the use of Albizia richardiana as traditional medicine.
Highlights
Albizia richardiana belongs Fabaceae family which different parts like fruits, flowers, barks, and roots are used medicinally
All the extracts exhibited excellent antioxidant activity in the DPPH scavenging assay and maximum total phenol content observed by N-hexane fraction of Albizia richardiana (HXFAR)
The results of the present study provided scientific evidence for the use of Albizia richardiana as traditional medicine
Summary
Albizia richardiana belongs Fabaceae family which different parts like fruits, flowers, barks, and roots are used medicinally. The study reports the in vitro anti-inflammatory, thrombolytic, cytotoxicity and antimicrobial activity of methanolic extract of A. richardiana stem and its different fractions. A. richardiana are used in treating depression, appetite, increase blood circulation, tightness in chest, eye problems, blurred vision and pain in back [6]. This plant reported to have several secondary metabolites in bark such as carbohydrates, saponins, glucosides, glycosides and alkaloids. It was reported to have significant antioxidant activity while lethal concentration (LC50) was extremely toxic in bark extract. The hypoglycemic and antimicrobial activity was evaluated [7]
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