Abstract
Previous studies have used digestion techniques with SEM/EDS to identify foreign material in lungs, These studies have identified elements with inclusions of lung tissue exposed to cigarette smoke, and clinical cases having ferruginous bodies. We have established a protocol to examine fixed lung tissue using x-ray microanalysis with STEM. Our technique allowed both localization and elemental analysis of foreign material in tissue. In the present study lung tissue from cigarette smokers and bronchial washings containing ferruginous bodies were examined.Histological slides of smoker lung tissue were examined to locate dark granular inclusions in cells and the interstitium. Cytology cell block slides of bronchial washings depicting ferruginous bodies were also selected. After locating these granules and bodies, tissues were carefully removed from the paraffin blocks, deparaffinized, and embedded in Spurr. Sections ranging in thickness from 180nm - 500nm were cut with a diamond knife and collected on nylon grids.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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