Abstract
The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500-nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections.
Highlights
The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells were cultured on the nanopillars
Description Analyzing cell behaviors and controlling them is vital for cell research, and there have been many efforts for developing novel methods and instruments to reveal complex correlations of the cell and its behaviors, and to control them (Stevens and George 2005)
* Correspondence: jongsoukyeo@yonsei.ac.kr 1School of Integrated Technology, Yonsei University, 85 Songdogwahak-ro, Yeonsu-gu, Incheon 21983, South Korea 2Yonsei Institute of Convergence Technology, Yonsei University, 85 Songdogwahak-ro, Yeonsu-gu, Incheon 21983, South Korea Full list of author information is available at the end of the article biocompatible PUA, the novel substrates of different pillar sizes and periodic arrays were fabricated to culture human adipose-derived stem cells (hASCs) on the nanopillars in order to understand the mechanotransduction of hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology
Summary
The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. Among them, investigating and controlling cell behavior via nanopillars become one of the high-interest topics.
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