Abstract
We perform stimulated emission depletion (STED) microscopy with a novel light source consisting of a fiber-amplified, frequency doubled laser operating with a 1 MHz repetition rate and a 530 nm output coupled into a standard single mode fiber to produce a tunable spectrum of discrete peaks via stimulated Raman scattering (SRS). Using peaks at 585, 600, and 616 nm as STED light we perform STED microscopy with resolution down to 20-30 nm. The nanosecond pulsed light source should prove valuable for all forms of microscopy requiring both brilliance and multiple wavelengths in the visible range.
Highlights
Stimulated emission depletion (STED) microscopy is a powerful tool for the sub-diffraction visualization of fluorescent structures in the far field [1], able to make observations#113471 - $15.00 USD Received 26 Jun 2009; revised Aug 2009; accepted Aug 2009; published 20 Aug 2009(C) 2009 OSA noninvasively on the nanoscale to explore subjects as diverse as endoplasmic reticulum dynamics in living cells [2] and the properties of single color-center defects in bulk diamonds [3]
We perform stimulated emission depletion (STED) microscopy with a novel light source consisting of a fiber-amplified, frequency doubled laser operating with a 1 MHz repetition rate and a 530 nm output coupled into a standard single mode fiber to produce a tunable spectrum of discrete peaks via stimulated Raman scattering (SRS)
The nanosecond pulsed light source should prove valuable for all forms of microscopy requiring both brilliance and multiple wavelengths in the visible range
Summary
Stimulated emission depletion (STED) microscopy is a powerful tool for the sub-diffraction visualization of fluorescent structures in the far field [1], able to make observations#113471 - $15.00 USD Received 26 Jun 2009; revised Aug 2009; accepted Aug 2009; published 20 Aug 2009(C) 2009 OSA noninvasively on the nanoscale to explore subjects as diverse as endoplasmic reticulum dynamics in living cells [2] and the properties of single color-center defects in bulk diamonds [3].
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