Abstract
Electroretinograms (ERG) or pattern-electroretinograms (PERG) could be valuable for the quantification of potential damage to the pig retina by experimental erbium:YAG laser treatment. We therefore performed a normative study of ERGs and PERGs in pigs. We recorded ERGs and PERGs under general anaesthesia in two experiments. In experiment 1 we examined eight eyes from six pigs of 20-25 kg body weight; in experiment 2 we examined four eyes from four pigs of 40-45 kg body weight. We used flash and checkerboard stimuli. In experiment 1, the stimulus parameters were mean luminance 48.3 cd/m2 for checkerboard stimuli, 96.6 cd/m2 for ERG, check sizes of 4 degrees, 8 degrees, and 16 degrees, temporal frequencies were 16 Hz for ERG and 8 rev/s for PERG. Three measurements were repeated after two weeks. Stimulus parameters for experiment 2 were luminance 175 (350) cd/m2, check sizes 1.6 degrees, 3.2 degrees, 6.7 degrees, and 16 degrees, temporal frequencies 6.3 Hz for ERG and 8 rev/s for PERG. Recordings were subjected to Fourier analysis. In experiment 1 the mean ERG amplitude was 1.02 +/- 0.89 microV with a coefficient of variation of 42% for repeat sessions. The mean PERG amplitudes were 0.53 +/- 0.25 microV for 16 degrees checks, 0.36 +/- 0.21 microV for 8 degrees, and 0.25 +/- 0.17 microV for 4 degrees. The mean coefficient of variation between two measurements was 103% for 16 degrees checks, 24% for 8 degrees, and 116% for 4 degrees. In experiment 2 the mean ERG amplitude was 9.72 +/- 3.96 microV. The mean PERG amplitudes were 0.77 +/- 0.50 microV for 16 degrees checks, 0.09 +/- 0.16 microV for 6.7 degrees, 0.07 +/- 0.13 microV for 3.2 degrees, and 0.08 +/- 0.09 microV for 1.6 degrees. It was possible to record ERGs and PERGs in pigs. However, the ERG amplitudes were small; PERG amplitudes were even smaller in both groups and cannot be reliably recorded. A problem for both ERG and PERG was the high intra-individual and interindividual variability. Therefore, only very extensive damage to the retina by vitrectomy or Er:YAG laser treatment might lead to a significant change in the ERG or PERG amplitudes.
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More From: Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie
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