Abstract
Reversible associations of macromolecules, by definition, create fragile complexes whose shape and stoichiometry are important to their function; further, any real system is likely to contain multiple species. Although true structural techniques like X-ray crystallography and solution NMR spectroscopy can give atomiclevel detail of the consensus forms of tight and rigid complexes, we also desire techniques applicable to dilute solution, dynamic equilibria, and flexible macromolecules. Fluorescence emission anisotropy has proven to be valuable in these areas. Fluorescence emission anisotropy allows us to directly measure Brownian rotations, and the hydrodynamics of those rotations depends on size, shape, and flexibility of the complex.
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