STATUS OF THE HUMORAL LINK OF IMMUNITY AT EXPERIMENTAL PERITONITIS ON THE BACKGROUND OF DIABETES MELLITUS

Abstract

Introduction. The combined course of acute peritonitis and diabetes increasingly leads to the development of secondary immunodeficiencies, which contribute to an increase in the percentage of postoperative complications and fatalities, the emergence of complexity of treatment and diagnosis.
 The aim of the study – to learn the peculiarities of humoral link of the immune response in the organism of experimental animals during simulated acute common peritonitis on the background of diabetes mellitus compared with animals with experimental acute peritonitis alone.
 Reserch Methods. 56 white rats were used in this work. Diabetes mellitus was modeled by intraperitoneal administration of Srethosotocin from “Sigma” firm with the rate of 7 mg per 100 g of animal mass, acute peritonitis – insertion of 0.5 ml of 10 % of the captured fecal suspension into the abdominal cavity of the animals. The concentration of immunoglobulins in serum was determined. Observation time points: 1, 3, and 7 days from the beginning of the peritonitis modeling.
 Results and Discussion. Analyzing the results of studies, it can be stated that there were observed directed changes in the concentration of immunoglobulins in blood serum of rats of different groups. Thus, on the first day after simulating acute common peritonitis on the background of diabetes, the level of Ig G decreased by 1.5 times, Ig M – by 1.3 times and Ig A – by 1.4 times (p<0.05). On the seventh day of the experiment, levels of all classes of immunoglobulins were statistically significantly lower than those of the group with acute common peritonitis: Ig G level was lower by 21 %, Ig M and Ig A were lower by 17 % and 56 % respectively (p<0.05). The results of our studies indicate that the presence of diabetes in acute general peritonitis leads to a decrease in the resistance of the humoral level of the immune system with antigenic stimulation and secondary immunodeficiency states.
 Conclusion. In animals with acute peritonitis, protective mechanisms of the humoral immunity level are substantially reduced on the background of diabetes, which is confirmed by statistically significantly lower levels of immunoglobulins of classes A, M, G compared to a group of animals with a pattern of acute peritonitis. The revealed functional impairment of humoral immunity indicates an appreciable weakening of the organism's protection factors in acute peritonitis on the background of diabetes mellitus with the development of secondary immunodeficiency.