Status of ESBL Producing Bacteria Isolated from Skin Wound at a Tertiary Care Hospital in Bangladesh

Taslima Yasmin,Mohammed Abdun Nur Sayam,Rezwana Haque,Md Abdullah Yusuf,Golam Mowla

doi:10.4236/aid.2015.54022

Taslima Yasmin, Mohammed Abdun Nur Sayam + Show 3 more

Open Access

https://doi.org/10.4236/aid.2015.54022

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Abstract

Background: ESBL producing bacteria are increasing with an alarming rate with a wide range of infections. Objective: The purpose of the present study was to see the status of ESBL producing bacteria isolated from skin wounds. Methodology: This cross sectional study was conducted in the Department of Microbiology at Mymensingh Medical College, Bangladesh from January 2011 to June 2011 for a period of 6 months. All the patients, at any age with both sexes presented with skin wound infection, were taken as study population. Wound swab was taken from all patients. Specimens were processed and bacteria were isolated and identified according to standard procedure. The ESBL status was confirmed by double disc diffusion test (DDDT) and minimum inhibitory concentration (MIC) by agar dilution method by standard procedure according to Clinical Laboratory Standard Institute (CLSI). Antimicrobial resistance was done by disc diffusion method. Result: A total number of 84 wound swabs were taken of which the most common ESBL producing bacteria were Esch. coli (61.5%), Proteus species (78.3%) and Klebsiella species (88.9%). All the isolates were sensitive to imipenem and nitrofurantoin followed by amikacin (92.9%). Conclusion: In conclusion, ESBL producing E. coli is the most common bacteria causing skin wound infection followed by Proteus species with a reduced sensitivity towards antibiotics.

Highlights

Skin infections are very frequently encountered in clinical practice and are one of the most common sites of bacterial infections [1]
All the patients at any age with both sexes presented with skin wound infection who were attended at the OPD as well as the patients who were admitted in the IPD were taken as study population
Two diagnostic tests were performed for phenotypic detection of ESBL producing bacteria which were disc diffusion test (DDT) [18] used as screening test for ESBL production and double disc diffusion test (DDDT) [18] as confirmatory test

Summary

Introduction

Skin infections are very frequently encountered in clinical practice and are one of the most common sites of bacterial infections [1]. Increased use of antibiotics, the third generation cephalosporin, has been associated with the emergence of β-lactamases [2]. These enzymes have serine at their active site and attack the amide bond in the beta lactam ring [3]. Objective: The purpose of the present study was to see the status of ESBL producing bacteria isolated from skin wounds. Result: A total number of 84 wound swabs were taken of which the most common ESBL producing bacteria were Esch. Conclusion: In conclusion, ESBL producing E. coli is the most common bacteria causing skin wound infection followed by Proteus species with a reduced sensitivity towards antibiotics

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