Abstract
The present investigation was undertaken to study status of erythrocytic antioxidant enzymes in normal cycling and α- tocopherol supplemented anestrus buffalo heifers. The pre-supplementation erythrocytic activities of superoxide dismutase (U/mg Hb), glutathione peroxidase (U/mg Hb) and glucose-6-phosphate dehydrogenase (U/g Hb) upregulated significantly (p<0.05) in anestrus heifers (10.08±0.09, 14.09±0.54, 9.25±0.29) when compared to normal cycling ones (6.93±0.04, 11.61±0.19, 5.58±0.26). The oral supplementation of α-tocopherol @ 3,000 mg per week per animal in anestrus heifers declined erythrocytic superoxide dismutase and glucose-6-phosphate dehydrogenase activities significantly (p<0.01) but led to non-significant increase in erythrocytic glutathione peroxidase activity. Results indicated that supplementation of α-tocopherol to anestrus buffalo heifers mitigated the effects of oxidative stress to improve their antioxidant status. (Asian-Aust. J. Anim. Sci. 2003. Vol 16, No. 2 : 217-221)
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